FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs7_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs7_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6044373
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT220870.365414245613234TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG37100.038.12640411
CACGTCT37650.037.33542314
GATCGGA34450.036.608691
ACGTCTG38450.036.55861315
CGTCTGA38550.036.46407716
ACACGTC38650.036.4263513
CACACGT38950.036.371712
AGCACAC40800.034.83119210
TCCAGTC40450.034.75389525
CAGTCAC40450.034.48195327
CACAGTC40350.034.12953631
GAACTCC41200.034.11926321
GAGCACA42000.033.993159
CTCGTAT38500.033.76935244
CCAGTCA41900.033.44618226
GTCACAG41650.033.33001329
AGAGCAC43550.032.934838
ACTCCAG43050.032.60302423
TCTCGTA39450.032.39851443
TCGGAAG43950.032.2474563
Produced by FastQC (version 0.11.2)