FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs6_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs6_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6117046
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC210530.3441693915657983TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91640.14981087276440294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34150.039.23058711
ACACGTC35550.037.87097513
ACGTCTG35900.037.50176215
CACACGT36050.037.4677712
CACGTCT36500.036.88529214
CGTCTGA37200.036.07294516
GATCGGA33700.035.660281
GAACTCC37850.035.1625721
CCAGTCA37700.035.07136526
CGTATGC37050.035.03119344
TCCAGTC38300.034.69426325
CAGTCAC38550.034.46927327
AGCACAC38950.034.39572510
TCGGAAG39950.033.6027343
TCGTATG38250.033.5295943
GAGCACA39900.033.5216529
AGTCACC39600.033.4997628
AGAGCAC40450.033.3380478
ACTCCAG40250.033.12138723
CTCGTAT34750.032.98177742
Produced by FastQC (version 0.11.2)