FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs5_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs5_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4968316
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC152770.30748849308296816TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29950.038.1948911
CGTCTGA30900.037.16316
ACGTCTG31250.036.8875715
CACACGT31150.036.723512
ACACGTC31750.036.3759513
GATCGGA29100.036.22821
CACGTCT31950.036.14824314
GAGCACA32450.035.4553159
AGCACAC33150.034.84005710
TCCAGTC32350.034.68158725
CGTATGC30150.034.65812344
CAGTCAC32600.034.4831127
GAACTCC32950.034.11682521
CCAGTCA33300.033.62611426
AGAGCAC34400.033.4454968
CATCTCG24650.033.02082439
AGTCACC34700.032.5230328
TCGGAAG35950.032.0147553
CTGAACT36800.031.503719
CCAGATC34850.031.30992333
Produced by FastQC (version 0.11.2)