FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs4_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs4_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6073565
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC464210.7643122284852472TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC58000.041.45918729
GCACACG60150.040.70543311
AGTCACG59700.040.64745728
TCACGCC59600.040.34552430
ACACGTC61200.040.00705313
ACGTCTG61700.039.6115415
CACACGT61900.039.5901712
GATCGGA58600.039.3248631
CACGCCA61550.039.17422531
CGTATGC60500.038.9794644
CACGTCT62800.038.84765214
CGTCTGA62950.038.75508516
AGCACAC63500.038.6619110
CAGTCAC63700.038.1986427
TATCTCG60350.037.7637739
GAACTCC64900.037.76167321
GAGCACA65450.037.610559
TCCAGTC65000.037.50235725
CCAGTCA65800.036.87922726
TCGTATG63350.036.77411343
Produced by FastQC (version 0.11.2)