FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs3_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs3_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4445495
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC71030.15977973206583293TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69240.15575318384116954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15450.035.5962211
CACACGT17100.032.93337612
ACGTCTG17250.032.0093615
ACACGTC17550.031.71288713
GTCACAC17500.031.67992629
CACACAG17900.030.971331
CGTCTGA18000.030.7978516
GATCGGA16600.030.6271341
CACGTCT18150.030.4221214
CGTATGC17800.029.90802644
AGCACAC18950.029.13780810
GATCTCG13750.029.11796839
CAGTCAC18650.029.018727
TCCAGTC18650.028.90073825
GAGCACA19950.027.6772659
CCAGTCA19600.027.3876926
GAACTCC19950.027.23619321
AGTCACA20600.026.9125628
TCGTATG19650.026.86835343
CACAGTG20650.026.63252333
Produced by FastQC (version 0.11.2)