FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs2_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs2_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7871001
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC83180.10567906165937471TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG19150.031.01628111
ACACGTC20500.029.40298513
ACGTCTG20700.029.11889815
CACACGT21350.028.12933512
GATCGGA19450.028.0638681
ACTGACC20900.027.99837532
CGTCTGA22200.026.95321816
CACGTCT22350.026.87075214
CAGTCAC23250.025.54901327
CGTATGC22750.025.04438244
TCCAGTC23850.024.81402625
AGCACAC25200.024.00623910
CCAGTCA25050.023.6253326
GAACTCC25250.023.17489221
CACTGAC26050.022.97060431
CTGACCA26100.022.92572833
GAGCACA26850.022.6959
AGAGCAC26750.022.6153668
TCGGAAG27450.022.3664633
TCGTATG25950.021.7017443
Produced by FastQC (version 0.11.2)