FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs1_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs1_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6841927
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80340.1174230593223225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13800.026.1430211
ACGTCTG14800.025.11960615
CACGTCT15050.023.8253314
CACACGT14950.023.69040712
CGTCTGA15400.023.14116
ACACGTC15650.022.4902113
GATCGGA14400.022.3150181
CCGATGT16050.021.6557433
AGCACAC17000.021.6101910
GAGCACA17900.020.5240929
CACCGAT17450.020.297731
CGTATGC17000.020.1869144
CAGTCAC17600.020.12529827
GTCACCG17400.019.7244329
TCCAGTC18950.019.0398625
ACCGATG18400.018.77050832
CCAGTCA19000.018.75817126
TATCTCG16700.018.70549639
CGATGTA18350.018.70163334
GAACTCC19000.018.64074521
Produced by FastQC (version 0.11.2)