FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs18_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs18_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6594263
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC140980.21379189759340808TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22850.036.4873911
CACGGCT22850.035.81619331
TCACGGC23650.034.88398730
GTCACGG23750.034.45973229
CACACGT24650.034.0014812
ACACGTC25000.033.70144313
CGGCTAC24500.033.67117333
ACGTCTG25700.033.04029515
CACGTCT26000.032.91288814
AGTCACG25150.032.89140328
ACGGCTA25400.032.3061132
CGTCTGA26400.032.16422716
GATCGGA25800.030.026891
CAGTCAC27550.029.86637927
GGCTACA26350.029.72070734
CATCTCG24200.029.36207839
CGTATGC27950.029.20037544
AGCACAC28850.029.05152310
TCCAGTC28850.028.52036325
GAGCACA29650.028.3418669
Produced by FastQC (version 0.11.2)