FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs17_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs17_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5836172
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC62000.10623401777740615TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG12750.027.95034411
CACACGT14200.024.94134512
CGTCTGA14500.024.88066116
ACACGTC14650.024.62569813
ACGTCTG14450.024.50983415
GATCGGA13350.024.402511
CACGTCT15650.022.34936114
TATCTCG13700.022.15852539
AGCACAC16500.021.86463710
CGTATGC15750.021.22977344
CAGTCAC16950.020.89642527
GAACTCC17700.020.63173521
TCCAGTC17200.020.46479225
CACTAGC17150.020.26755531
GAGCACA18150.020.2407199
CCAGTCA17300.020.21933426
ACTAGCT18400.019.1291532
TCACTAG19050.018.4772430
TCGGAAG19650.018.1450463
AGTCACT20050.018.10445228
Produced by FastQC (version 0.11.2)