FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs16_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs16_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4946764
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC241160.4875106231063378TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38850.040.9961711
CACACGT39750.040.0675512
CGATCAG39850.039.5253833
ACACGTC40400.039.4773513
GTCACGA39850.039.4185629
CACGTCT40800.039.09031714
AGTCACG40700.038.86559728
CACGATC41000.038.4723431
ACGTCTG41450.038.42424815
GATCGGA38450.038.293331
GATCTCG34300.037.7111839
CGTCTGA42450.037.4154416
AGCACAC42950.037.23671710
CCAGTCA42850.036.96685826
CAGTCAC42850.036.91551627
CGTATGC42300.036.76803244
TCCAGTC42850.036.76148625
AGAGCAC43700.036.7486658
GAGCACA43950.036.739849
TCACGAT43400.036.2441930
Produced by FastQC (version 0.11.2)