FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs15_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs15_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5236099
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG9500.024.77714511
CACACGT10650.023.13446812
GATCGGA10100.021.5743261
ACACGTC10950.021.29525613
CACGTCT11450.019.78895614
ACGTCTG11700.019.74215515
GTCACAC11350.019.38314429
CGTCTGA12500.018.83063116
TCCAGTC12150.018.65009725
CAGTCAC12250.018.3182627
GAGCACA13400.018.0583889
AGTCACA13150.016.8972428
AGCACAC14050.016.75339110
AGAGCAC14750.016.704038
GAACTCC14550.016.3287121
CCAGTCA14050.016.1280226
TCGGAAG15550.015.5675163
CGTATGC12450.015.19597244
ACTCCAG15700.014.71286923
TCTGAAC17200.014.58033618
Produced by FastQC (version 0.11.2)