FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs14_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs14_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4023206
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC6750.026.07161513
ACTTAGG6950.025.63946232
GCACACG7050.024.65046311
ACGTCTG7350.024.54190315
CACACGT7500.023.75775712
CTTAGGC7600.023.44515633
CACGTCT7800.022.84399814
CGTCTGA8400.021.21228416
GATCGGA8050.021.0545771
CGTATGC8750.020.86660444
AGCACAC8900.019.7736610
CACTTAG9200.019.60855131
CAGTCAC9450.019.3233327
GAACTCC9350.019.29325721
CCAGTCA10100.018.29757726
GAGCACA10050.017.9490019
TCCAGTC10300.017.94228625
TTAGGCA9900.017.331734
CATCTCG7650.017.25327539
AGAGCAC10450.017.0514478
Produced by FastQC (version 0.11.2)