FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs13_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs13_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4855333
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG8550.027.78761311
CACACGT8750.027.4038812
ACACGTC10400.023.26767313
ACGTCTG10450.022.94583115
GATCGGA10000.021.791561
CGTCTGA11650.020.95996916
GAGCACA11600.020.2913289
AGCACAC12200.019.83453210
TCCAGTC12150.019.73652525
CGATCTC8150.019.70379638
CACGTCT12250.019.574214
GATCTCG8650.019.07347539
CACGATC8900.018.53770336
CAGTCAC12700.018.53533727
GAACTCC13300.018.35986121
CGTATGC12800.018.21715544
CCAGTCA13200.018.16657326
CATCACG12500.017.77462633
AGAGCAC13350.017.6315968
TCGGAAG14650.016.675043
Produced by FastQC (version 0.11.2)