FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs12_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs12_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4992126
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT3594157.199637989906504TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT301450.6038509444673472TruSeq Adapter, Index 19 (97% over 44bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT244610.489991638832834TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT235120.47098170198428485TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTAT76240.15272050425009306TruSeq Adapter, Index 19 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT73290.14681119827504352TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT67880.13597413206317308TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58150.11648343811834878No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGTAT55320.11081451069143687TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA497350.043.5600551
GCACACG550950.043.3261411
ACACGTC551600.043.28705213
CACACGT551950.043.27155712
AGCACAC552850.043.1732610
TCGGAAG552750.043.15063
CACGTCT552850.043.14142614
ACGTCTG552250.043.13651315
GAGCACA553500.043.119029
AGAGCAC555100.043.0867468
CGTCTGA551700.043.08026516
ATCGGAA555400.043.044212
CTCGTAT497100.043.02378544
AGTCACG548000.042.99106228
CACGTGA547650.042.94092631
GTCACGT547600.042.93810329
GAACTCC548300.042.9341521
TCACGTG548850.042.9111730
CGGAAGA556550.042.8282974
TCCAGTC550150.042.7910625
Produced by FastQC (version 0.11.2)