FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs11_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs11_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4049954
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT128440.3171394045463232TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG20300.037.8200832
CGTCCGC20700.036.98298333
GCACACG21850.036.54670711
CACGTCC21200.036.42249331
GATCGGA21500.036.3397031
AGTCACG21700.035.99010528
CACACGT22250.035.98855612
CCGCACA19100.035.93472336
ACACGTC22450.035.4719713
GTCACGT22050.035.31906529
TCCGCAC21000.035.30235735
GTCCGCA21350.035.2388134
CGTCTGA23000.034.33679216
CACGTCT23200.034.23042314
ACGTCTG23850.033.3897615
TCACGTC23600.033.0921930
TCCAGTC23750.033.0688525
CATCTCG19950.032.7496541
AGCACAC24650.032.57384510
CAGTCAC24300.032.41091527
Produced by FastQC (version 0.11.2)