FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs10_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs10_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6796567
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1889402.7799328690499188TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT222330.32712103036724277TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT200110.29442805463405275TruSeq Adapter, Index 16 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109990.16183170121033164No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATGTCGTAT69650.10247820701245203TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG321950.043.18358211
GATCGGA314550.042.9618381
CGTCCCG309600.042.9447735
ACGTCTG325050.042.71759815
GAGCACA326000.042.7152069
ACACGTC325900.042.7006813
AGCACAC325650.042.6996910
GTCCCGA279150.042.66457736
CACGTCT326350.042.59461614
CACACGT326550.042.5685312
CCCGTCC314250.042.53363433
CGTCTGA325000.042.500816
ACCCGTC311600.042.41623332
TCACCCG312900.042.38186330
AGAGCAC329900.042.3769458
CCCGATC229650.042.27245738
CCGTCCC314450.042.24773434
GTCACCC314900.042.2187229
TCCAGTC323700.042.1584125
CACCCGT314700.042.0622531