Basic Statistics
Measure | Value |
---|---|
Filename | HWKFWAFXX_n01_vs10_180118.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 6796567 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT | 188940 | 2.7799328690499188 | TruSeq Adapter, Index 16 (97% over 40bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT | 22233 | 0.32712103036724277 | TruSeq Adapter, Index 16 (97% over 40bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT | 20011 | 0.29442805463405275 | TruSeq Adapter, Index 16 (97% over 44bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 10999 | 0.16183170121033164 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATGTCGTAT | 6965 | 0.10247820701245203 | TruSeq Adapter, Index 16 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 32195 | 0.0 | 43.183582 | 11 |
GATCGGA | 31455 | 0.0 | 42.961838 | 1 |
CGTCCCG | 30960 | 0.0 | 42.94477 | 35 |
ACGTCTG | 32505 | 0.0 | 42.717598 | 15 |
GAGCACA | 32600 | 0.0 | 42.715206 | 9 |
ACACGTC | 32590 | 0.0 | 42.70068 | 13 |
AGCACAC | 32565 | 0.0 | 42.69969 | 10 |
GTCCCGA | 27915 | 0.0 | 42.664577 | 36 |
CACGTCT | 32635 | 0.0 | 42.594616 | 14 |
CACACGT | 32655 | 0.0 | 42.56853 | 12 |
CCCGTCC | 31425 | 0.0 | 42.533634 | 33 |
CGTCTGA | 32500 | 0.0 | 42.5008 | 16 |
ACCCGTC | 31160 | 0.0 | 42.416233 | 32 |
TCACCCG | 31290 | 0.0 | 42.381863 | 30 |
AGAGCAC | 32990 | 0.0 | 42.376945 | 8 |
CCCGATC | 22965 | 0.0 | 42.272457 | 38 |
CCGTCCC | 31445 | 0.0 | 42.247734 | 34 |
GTCACCC | 31490 | 0.0 | 42.21872 | 29 |
TCCAGTC | 32370 | 0.0 | 42.15841 | 25 |
CACCCGT | 31470 | 0.0 | 42.06225 | 31 |