FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_ah7797i3hf30min.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_ah7797i3hf30min.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6446001
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT447370.6940271960863797TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG66650.040.20042411
ACACGTC67600.039.7005613
AGTCACG65400.039.69337528
CACGTGG64100.039.5711231
ACGTGGC64450.039.35561432
ACGTCTG68450.039.11115315
GTCACGT66450.039.03306229
CACACGT69050.038.8350212
CGTGGCC65050.038.78849833
GGCCTTA63450.038.72651336
GATCGGA66000.038.318541
CGTCTGA69550.038.302816
AGCACAC70400.038.18405510
TCACGTG67800.038.0611730
TATCTCG60200.037.9669741
CACGTCT70800.037.93725214
GAGCACA71600.037.6669969
CAGTCAC69950.037.20581427
GAACTCC70550.037.0756321
TCCAGTC70750.036.94058225
Produced by FastQC (version 0.11.2)