FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_ah7797hac3hf30min.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_ah7797hac3hf30min.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6647020
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT162050.24379345932462967TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32600.037.0465111
ACACGTC32800.036.88740513
AGTCACG32350.036.38297328
GATCGGA31050.035.9394071
CACACGT33650.035.89025512
GTCACGT33750.035.00412429
CACGTCT34550.034.9553414
CGTTTCG33550.034.8167333
CGTCTGA35000.034.75732816
ACGTCTG35450.034.5643415
GTTTCGG34350.033.94182234
AGCACAC37050.032.71567510
CAGTCAC37150.032.1558327
GAGCACA38000.031.8401369
CCAGTCA37500.031.79704326
AGAGCAC38700.031.3779058
TCCAGTC39000.030.63048725
TCGGAAG40650.029.9942363
CACGTTT39900.029.60808231
TCACGTT40250.029.51459130
Produced by FastQC (version 0.11.2)