FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_ah7669i3hf5min.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_ah7669i3hf5min.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5942666
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1713412.8832345617270096TruSeq Adapter, Index 25 (97% over 44bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT150790.2537413342765688TruSeq Adapter, Index 25 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG223950.042.70916711
CACACGT224400.042.68234312
GATCGGA198750.042.616821
ACGTCTG225100.042.5105215
ACACGTC225350.042.4731213
CGTCTGA225600.042.4264116
CACGTCT225950.042.350614
GAGCACA226400.042.3150029
GTCACAC223500.042.306629
GAACTCC224900.042.0896721
CACACTG224650.042.0312731
TCCAGTC224950.041.9947725
AGCACAC228300.041.9435710
CTCGTAT217500.041.87376444
CAGTCAC226600.041.7472427
TATCTCG216850.041.72501841
TCGGAAG230650.041.658093
AGAGCAC230800.041.613858
CCAGTCA227250.041.54070326
AGTCACA227800.041.50800728
Produced by FastQC (version 0.11.2)