FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_ah7669i3hf30min.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_ah7669i3hf30min.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6573705
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT463030.704366867694854TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG64850.040.09596333
GCACACG66750.040.01017811
ACGTCTG67900.039.3319415
ACACGTC68300.039.26263813
CGTACGT66200.038.9127634
CACACGT68950.038.8922112
CACGTCT68650.038.87019714
GATCGGA64050.038.587391
AGCACAC69650.038.40716610
CGTCTGA69650.038.3121216
ACCGTAC68350.037.9470832
GTACGTA68450.037.76222635
CAGTCAC69100.037.6639927
GAACTCC70400.037.5605221
GAGCACA71400.037.5582479
GTCACCG69050.037.53167329
TCCAGTC70650.037.02450625
CTCGTAT67100.036.71909744
AGTCACC70450.036.69243228
CCAGTCA71450.036.36363226
Produced by FastQC (version 0.11.2)