FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_ah7669hac3hf5min.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_ah7669hac3hf5min.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6010186
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT572430.9524330860975018TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG83950.041.06160411
ACACGTC84200.040.9393513
CACACGT85350.040.46505712
ACGTCTG86000.040.21037315
GATCGGA75850.040.1041641
CGTCTGA87000.039.64737316
CACGTCT87200.039.60656414
AGCACAC87700.039.3810910
CAGTCAC86450.039.16460427
TCCAGTC86900.039.0883825
GAGCACA88600.039.0558629
CTCGTAT83550.038.73121344
AGTCACA88150.038.6339228
TATCTCG80800.038.6333441
TCGGAAG89700.038.5451853
GTCACAT88200.038.4623629
AGAGCAC90150.038.384678
CCAGTCA88500.038.3071226
ACTCCAG90100.037.77210623
GTCTGAA91900.037.60523217
Produced by FastQC (version 0.11.2)