FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_ah7669hac3hf30min.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_ah7669hac3hf30min.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6375936
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT261910.41077890367782866TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82620.1295809744639846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG36450.038.50929628
GCACACG37700.037.9863511
CACGAGT37000.037.57890731
CGAGTGG37550.037.25933533
ACGAGTG37900.036.97532732
GTCACGA37850.036.96865529
CACACGT39250.036.71014812
ACACGTC39300.036.3835713
TCACGAG38750.036.3362830
ACGTCTG39800.036.2028515
CGTCTGA40500.035.68575316
GATCGGA37000.035.512841
CACGTCT40500.035.3055414
AGCACAC41050.035.04713410
TATCTCG39100.034.76932541
GAGCACA42450.034.046489
CAGTCAC41650.033.9655327
TCCAGTC43250.032.8616125
CCAGTCA43600.032.54734826
CTCGTAT42550.032.3637844
Produced by FastQC (version 0.11.2)