FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19000462
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1167960.6147008425374078TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC158950.057.73427246
TATGCCG160300.056.65898548
GTATGCC164650.056.22492247
ATGCCGT163000.055.1621849
CGTATCT171450.054.91271239
GCCGTCT157650.054.63646351
CCGTATC182250.054.61617338
TGCCGTC164450.054.0798850
TCGTATG171500.053.14205645
CTCGTAT172300.052.77343844
GTTCCGT198350.052.00045435
TCCGTAT199700.051.36837437
GTATCTC184150.050.66948740
AGTTCCG208850.049.7212834
CCGTCTT179700.048.98406652
GTCACAG218550.048.73156429
TATCTCG197550.047.26796341
TTCCGTA221450.046.62357336
TCTCGTA198850.045.9735443
CAGTCAC233550.045.93141627
Produced by FastQC (version 0.11.2)