FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19040438
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT5411672.842198272959897TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG386360.2029155001581371No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTAT202290.1062423038797742TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC654100.065.889146
TATGCCG660100.065.0360448
GTATGCC661800.064.9904447
ATGCCGT660000.064.9716549
TGCCGTC657250.064.92416450
GCCGTCT653150.064.919151
TCGTATG664150.064.73923545
CTCGTAT640350.064.4344544
CCGTCTT670000.063.3597352
CTGCTTG662000.062.4178859
CACAGTC702700.062.21848331
TCTCGTA666450.061.9687943
AGTCAAC704150.061.4940834
GTCACAG721950.060.86976229
GTCAACA714350.060.55722435
ATCTCGT675000.060.52534542
CAATCTC673750.060.4298540
ACAGTCA722750.060.42967632
AATCTCG679800.060.05164341
CTTCTGC696900.059.95499456
Produced by FastQC (version 0.11.2)