FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20830143
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4384172.1047239090005285TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC543550.065.4723844
TATGCCG547000.065.0914346
GTATGCC550550.064.83745
ATGCCGT545550.064.8028347
TGCCGTC543200.064.773948
GCCGTCT540500.064.3008449
CTCGTAT529100.063.82055742
TCGTATG552750.063.81911543
CCGTCTT563700.062.12631650
CCTTGTA587600.061.70720733
CTGCTTG547600.061.06424757
GTAATCT556600.060.48486337
TCTCGTA558000.060.40852741
CACCTTG603800.060.22520431
ACCTTGT602700.060.16686232
TGTAATC565750.060.16238836
GTCACCT609950.059.8530829
AGTCACC619750.059.38100428
ATCTCGT573050.058.90127640
TAATCTC570950.058.87870438
Produced by FastQC (version 0.11.2)