FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20337357
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC811300.3989210594080637TruSeq Adapter, Index 11 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG383470.18855449112684602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC115300.055.1549844
ACGGCTA117450.053.48922332
CGGCTAC117600.053.18290733
TATGCCG121800.051.89561546
GTATGCC122150.051.6896145
ATGCCGT123300.051.2365247
CACGGCT123000.051.1609231
GTCACGG125600.050.4362429
GCCGTCT124950.050.3919949
TGCCGTC126500.050.07888448
TCACGGC127200.049.63673430
GGCTACA126400.049.14803334
TCGTATG130350.048.7063243
AGTCACG135850.046.99134428
CTCGTAT132100.046.94831542
GCTACAT140400.044.02287735
CCGTCTT149350.042.27638650
CAGTCAC156500.041.1934327
CTGCTTG155600.040.03832257
CCAGTCA162500.039.54321726
Produced by FastQC (version 0.11.2)