FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13436168
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2430101.8086257927111362TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC289330.21533669421221882TruSeq Adapter, Index 10 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243540.18125703697661416No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATTC169860.12641997331381982TruSeq Adapter, Index 10 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC435050.065.92728431
ACTAGCT438650.065.2346232
GTATGCC315600.065.1074245
CGTATGC307900.064.68957544
TAGCTTA442350.064.44369534
TATGCCG313900.064.1780546
TCACTAG449800.064.107530
CTAGCTT446750.063.88733733
CAGTCAC454950.063.59720627
GCTTATC432550.063.53336
CTCGTAT304300.063.14308542
CCAGTCA459000.062.93692826
TCGTATG290150.062.77259443
ATGCCGT299300.062.60806747
ACTCCAG463950.062.19023523
GCCGTCT281500.062.11611649
ACGTCTG469400.062.0606615
TCCAGTC465500.062.04330425
AGCTTAT458600.061.6336235
ACACGTC473900.061.6049913
Produced by FastQC (version 0.11.2)