FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18036313
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC9360365.189730295764994TruSeq Adapter, Index 9 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG631910.35035431021850194No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC271780.15068489884822914TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1095650.068.12659544
TATGCCG1098600.067.6985446
GTATGCC1099350.067.6873745
ATGCCGT1099100.067.5948747
TGCCGTC1099750.067.4308148
TCGTATG1106700.067.38312543
GCCGTCT1098350.067.3124549
CTCGTAT1066950.067.2627242
AGTCACG1113450.067.2131528
GTCACGA1113850.066.9564929
CACGATC1110300.066.9213731
CGATCAG1109100.066.8202133
GATCTCG1047200.066.6630239
CCGTCTT1112250.066.4682750
CAGTCAC1128350.066.46535527
CTGCTTG1110000.066.2565257
ACGATCA1126200.065.998332
CCAGTCA1136700.065.9432526
TCACGAT1131350.065.8403630
CAGATCT1057150.065.82368537
Produced by FastQC (version 0.11.2)