FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20115882
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG280900.13964090662293605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC50250.026.74573944
TATGCCG54700.025.14575646
GTATGCC56500.024.53048745
ATGCCGT52550.024.50968747
TGCCGTC57750.022.90880848
GTCACAC82500.022.73860529
GCCGTCT58050.021.46399149
ACGTCTG97250.021.01820615
CAGTCAC97150.020.85877227
CCAGTCA98550.020.8465626
CTCGTAT63400.020.81178742
TCGTATG62700.020.43011543
ACACGTC103750.019.73538213
CACGTCT106600.019.20755814
CGTCTGA107300.019.11458616
TCCAGTC107250.019.09024625
CACACGT107400.019.03246312
GCACACG107800.018.89713911
ACTCCAG108050.018.85177623
GAACTCC109700.018.69588921
Produced by FastQC (version 0.11.2)