FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21494488
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC1644380.7650240377905256TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG351450.16350703491983617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC229450.058.6496944
ATGCCGT227100.057.1611747
TATGCCG236900.056.79050446
GTATGCC240000.056.65485845
TGCCGTC231750.056.58813548
GCCGTCT220450.056.2341249
CTCGTAT227400.055.89986842
AGTCACC299750.054.5152528
TCGTATG233050.054.49971843
CCGTCTT247500.051.44568350
GTCACCA317550.051.4263829
ACCAGAT318250.050.91748432
CAGTCAC324050.050.8160527
CCAGTCA327900.050.1126726
CATCTCG274150.049.30368839
CCAGATC326150.049.21233
TCACCAG333350.048.93639830
TCCAGTC336500.048.80084225
ACTCCAG343000.048.0086923
CTGCTTG241800.047.9547557
Produced by FastQC (version 0.11.2)