FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19467594
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC2712651.393418210796876TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG369850.18998238816774174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC322750.064.0017444
GTATGCC326850.063.07040845
GTCACGC330050.062.74457629
TATGCCG330300.062.4858146
ATGCCGT330250.062.3576647
TCACGCC332950.061.96681230
TCGTATG333500.061.9177343
TGCCGTC333350.061.7357648
AGTCACG338150.061.6452628
GCCGTCT332150.061.5373149
CACGCCA334650.061.5369831
CTCGTAT332100.061.31456842
CCGTCTT347800.059.01989450
CAGTCAC357700.058.68699627
CTGCTTG344050.058.43227857
ACGCCAA354700.058.19694532
CCAGTCA364150.057.5225626
TATCTCG357600.057.15747539
CGCCAAT361400.057.1083533
TCTCGTA362000.056.4628941