FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20230030
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC3901401.9285191371441368TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC766530.3789070011265431TruSeq Adapter, Index 5 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG437660.21634174541510812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC568800.066.01108644
GTATGCC577550.065.36854645
TATGCCG576750.065.1315246
ATGCCGT568800.064.88586447
TCGTATG560700.064.4363543
TGCCGTC570050.064.344548
GCCGTCT559050.063.98907549
CTCGTAT479300.063.6597642
GATCTCG483700.062.6540839
CCGTCTT584500.062.292750
CACAGTG648350.062.16000733
GTCACAC651850.062.0302829
CTGCTTG551050.061.87586657
CAGTCAC661150.061.7612127
ACACAGT660450.061.22785632
CACACAG663300.061.01226431
AGTGATC521950.060.95936636
CCAGTCA671150.060.71568326
AGTCACA678050.060.0876528
TCTCGTA507550.059.89597341
Produced by FastQC (version 0.11.2)