FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13993764
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3915092.797739050051151TruSeq Adapter, Index 4 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205970.1471869898620557No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC192840.137804239088211TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC484350.065.6196644
GTATGCC486950.065.18304445
TATGCCG486900.065.1394246
ATGCCGT487150.065.070347
GCCGTCT484050.064.9666649
TCGTATG490650.064.7271643
TGCCGTC489800.064.5610448
CTCGTAT462900.064.554842
ACTGACC494150.063.22686432
CCGTCTT499950.063.04752750
CTGACCA494250.063.00163733
CAGTCAC513700.062.83730727
CTGCTTG494400.062.58012857
CCAGTCA515950.062.48187626
GTCACTG513950.062.39134629
CACTGAC509050.061.89185331
TCTCGTA480150.061.8421941
TCCAGTC524150.061.53777325
ACTCCAG525400.061.4846323
CCAATCT473650.061.46357737
Produced by FastQC (version 0.11.2)