FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18065351
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC378770.20966656003528522TruSeq Adapter, Index 3 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233140.12905367850311902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG71850.045.9837231
CGTATGC69800.043.02213344
ACTTAGG80050.041.09844232
ATGCCGT73950.039.6140947
TGCCGTC76550.038.0858348
TATGCCG79200.037.82758746
CTCGTAT76850.037.34458542
GCCGTCT76100.037.2532449
GTATGCC81500.037.01772345
TCGTATG81500.035.4717443
AGGCATC87250.035.29991536
GGCATCT88450.032.72382737
TTAGGCA104650.031.27006734
CCGTCTT97600.029.4772450
CCAGTCA116900.029.25079326
CAGTCAC117450.029.05430227
TCACTTA119950.027.9236230
TCCAGTC122350.027.89070325
ACTCCAG125850.027.36533223
CTGCTTG103350.026.65188257
Produced by FastQC (version 0.11.2)