FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17712979
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC445016325.123741184359783TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2516041.4204499423840564No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC2452941.3848263468273745TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATTC1334000.7531200708813577TruSeq Adapter, Index 2 (98% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG925130.5222893337139959No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATGC767280.43317388904486365TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTTTGC640920.361836368687616TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC530160.29930594960904094TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGG430310.2429348558477939TruSeq Adapter, Index 2 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAAGC367710.20759353917824888TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTATGC293710.16581626388198167TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATGC269200.15197895283452884TruSeq Adapter, Index 2 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAGCTCGTATGC224420.12669805570254444TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGC211390.11934186790375577TruSeq Adapter, Index 2 (98% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192210.10851364979318273No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATTC182740.10316728767080907TruSeq Adapter, Index 2 (97% over 48bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC5159950.068.86365545
ATGCCGT4937250.068.8497247
GCCGTCT4715600.068.8417249
TATGCCG5104900.068.8356546
CGTATGC5092750.068.83562544
TGCCGTC4909200.068.8174948
CTCGTAT4958200.068.8019542
TCGTATG4884050.068.7221643
GTATCTC5238450.068.6822338
CCGTCTT4861850.068.67155550
CTGCTTG4408100.068.6331757
TATCTCG5213500.068.5832839
TCTCGTA5003800.068.5722441
TGTATCT5356900.068.567637
ATGTATC5797050.068.4945136
CGATGTA5967750.068.4888434
CTTCTGC4684950.068.4484854
ATCTCGT5108350.068.4335640
GATGTAT5939300.068.4150335
TGCTTGA4534200.068.3826658
Produced by FastQC (version 0.11.2)