FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_las83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_las83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12014281
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC120385610.020208450260153TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG763980.6358932340603654No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC322960.2688134229588937TruSeq Adapter, Index 1 (98% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266250.22161126412808224No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC229000.19060649571955243TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC157200.13084428439787615TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1419900.068.3025844
GTATGCC1419500.068.230645
TATGCCG1419700.068.1790746
ATGCCGT1419050.068.11497547
GCCGTCT1413050.068.0970649
TCGTATG1421800.068.0485543
CTCGTAT1365700.068.0197342
TGCCGTC1420400.067.9368848
CACGATC1307100.067.8295636
ACGATCT1310550.067.7765237
GATCTCG1318800.067.7612239
CGATCTC1313900.067.7608738
CTGCTTG1411650.067.5177757
CATCACG1418700.067.47935533
CCGTCTT1425100.067.47488450
TCACGAT1369200.067.3221135
TCTCGTA1358950.067.3173841
ATCACGA1395500.067.14654534
CAGTCAC1450700.067.07355527
TGCTTGA1428400.066.84637558