Basic Statistics
Measure | Value |
---|---|
Filename | HWK5TBGX5_n01_las83.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12014281 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 1203856 | 10.020208450260153 | TruSeq Adapter, Index 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 76398 | 0.6358932340603654 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC | 32296 | 0.2688134229588937 | TruSeq Adapter, Index 1 (98% over 50bp) |
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 26625 | 0.22161126412808224 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC | 22900 | 0.19060649571955243 | TruSeq Adapter, Index 1 (98% over 50bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC | 15720 | 0.13084428439787615 | TruSeq Adapter, Index 1 (98% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 141990 | 0.0 | 68.30258 | 44 |
GTATGCC | 141950 | 0.0 | 68.2306 | 45 |
TATGCCG | 141970 | 0.0 | 68.17907 | 46 |
ATGCCGT | 141905 | 0.0 | 68.114975 | 47 |
GCCGTCT | 141305 | 0.0 | 68.09706 | 49 |
TCGTATG | 142180 | 0.0 | 68.04855 | 43 |
CTCGTAT | 136570 | 0.0 | 68.01973 | 42 |
TGCCGTC | 142040 | 0.0 | 67.93688 | 48 |
CACGATC | 130710 | 0.0 | 67.82956 | 36 |
ACGATCT | 131055 | 0.0 | 67.77652 | 37 |
GATCTCG | 131880 | 0.0 | 67.76122 | 39 |
CGATCTC | 131390 | 0.0 | 67.76087 | 38 |
CTGCTTG | 141165 | 0.0 | 67.51777 | 57 |
CATCACG | 141870 | 0.0 | 67.479355 | 33 |
CCGTCTT | 142510 | 0.0 | 67.474884 | 50 |
TCACGAT | 136920 | 0.0 | 67.32211 | 35 |
TCTCGTA | 135895 | 0.0 | 67.31738 | 41 |
ATCACGA | 139550 | 0.0 | 67.146545 | 34 |
CAGTCAC | 145070 | 0.0 | 67.073555 | 27 |
TGCTTGA | 142840 | 0.0 | 66.846375 | 58 |