FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_jk04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_jk04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8836751
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT202620.22929241754124338TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133510.1510849406076962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC38650.047.0880946
TATGCCG40950.044.52955648
GTATGCC41250.044.20570847
GCCGTCT41850.043.15353451
TGCCGTC42350.042.8919850
ATGCCGT42550.042.44360449
TCGTATG44000.042.0784945
AGTCACG44700.041.65443828
CTCGTAT43050.040.81221844
GTCACGT47750.038.48071329
GATCTCG46400.035.451741
CGATCTC45950.035.41805340
CCGTCTT51850.034.9657752
CAGTCAC53900.034.8692627
ACGATCT47300.034.33318339
TCACGTG54100.033.89933830
CCAGTCA56750.033.2414626
CTGCTTG54150.033.0933759
ACGTCTG57250.033.01339715
CACGTGA56100.032.62823531
Produced by FastQC (version 0.11.2)