FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_jk03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_jk03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8323989
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1871662.248513302936849TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC224900.066.6991733
ACGTCCG228850.065.9914532
GTCCGCA227350.065.6879134
CGTATGC233000.065.2220246
TATGCCG232450.065.106548
TCCGCAC228700.065.0859135
CACGTCC233000.064.9955331
GTATGCC234100.064.9013847
AGTCACG238700.064.7044328
TGCCGTC232550.064.7026250
ATGCCGT234700.064.3783449
GCCGTCT231700.064.29005451
CTCGTAT229400.064.0180544
GTCACGT242000.063.7642529
CGCACAT226450.063.7388337
TCGTATG237050.063.70906445
CCGCACA227800.063.59155736
ACGTCTG249150.063.24304615
TCACGTC242150.063.17553330
CAGTCAC246300.063.09153727
Produced by FastQC (version 0.11.2)