FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_jk02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_jk02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9685822
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT2045372.1117154537838916TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT103440.1067952725127511TruSeq Adapter, Index 16 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG260850.067.2342635
CCGTCCC263250.066.7409534
CCCGTCC265050.066.6042833
GTCCCGA250850.066.4826136
CACCCGT268100.066.264331
CGTATGC268200.066.16231546
ACCCGTC267800.066.1555632
TCACCCG269200.066.0325430
GTCACCC270950.065.799829
GTATGCC270150.065.4904247
CCCGATC235200.065.4302338
TGCCGTC267850.065.3990950
TATGCCG269600.065.3773648
GCCGTCT266500.065.375851
ATGCCGT269250.065.31901649
CTCGTAT257100.064.4447844
AGTCACC279550.064.3639928
TCGTATG274950.064.1943445
CGATCTC241900.063.488140
CCGATCT241100.063.4516539
Produced by FastQC (version 0.11.2)