FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_jk01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_jk01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11914017
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1264701.0615227424973457TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT126800.10642925891410093TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC133050.059.7130446
CAGTCAC266650.058.73671727
TATGCCG130150.058.51650648
CCAGTCA268050.058.3124226
CTCGTAT158850.058.05660244
ACATGTC264500.058.02301832
ATGTCAG266250.057.51019334
GTATGCC140200.057.44235647
CATGTCA267400.057.28903633
ATGCCGT130850.057.24030349
TCCAGTC274000.056.94372625
ACTCCAG277000.056.59210223
TCGTATG148150.056.5562645
AGTCACA276400.056.5254928
TGCCGTC129500.056.4588950
CACATGT273800.056.25694731
GCCGTCT116350.056.19219651
GAACTCC281550.055.7769921
GTCACAT278850.055.70252229
TCACATG280050.055.0389230
Produced by FastQC (version 0.11.2)