FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_dj13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_dj13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18953121
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACT358154718.896871918878162No Hit
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACC244596112.905320448278676No Hit
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACA14517237.659545886928068No Hit
CATTTTGACTGAACTGGAGAACAGTGTTCGAAATATATTTCAACTGTACG3603431.9012330475809234No Hit
CATTTTGACTGAACTGGAGAACAGATCAAAATTAGTTTACCTCGTTAGAT2141641.1299669326228647No Hit
AACGAGGTAAACTAATTTTGATCTGTTCTCCAGTTCAGTCAAAATGAGAT2085701.1004520047120472No Hit
CATTTTGACTGAACTGGAGAACACAGATCAAAATTAGTTTACCTCGTTAG1705970.9000997777621955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1649390.8702471745946221No Hit
AACGAGGTAAACTAATTTTGATCTGTGTTCTCCAGTTCAGTCAAAATGAG1561040.8236321606346522No Hit
CATTTTGACTGAACTGGAGAACACGATCAAAATTAGTTTACCTCGTTAGA1534190.8094656283785662No Hit
AACGAGGTAAACTAATTTTGATCGTGTTCTCCAGTTCAGTCAAAATGAGA1368070.7218177945468718No Hit
AACGAGGTAAACTAATTTTGATCTAGTTTGGTTAAGCGCTTAATTTGAAG1262940.666349357448834No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1130080.5962500846166707No Hit
CATTTTGACTGAACTGGAGAACACAAAGAAGATCAAAATTAGTTTACCTC843070.4448185604893252No Hit
GACGAGGTAAACTAATTTTGATCTAGTTTGGTTAAGCGCTTAATTTGAAG731480.38594171376840786No Hit
AACGAGGTAAACTAATTTTGATCTTCTTTGTGTTCTCCAGTTCAGTCAAA703790.37133198273782986No Hit
CATTTTGACTGAACTGGAGAACACAAAGATCAAAATTAGTTTACCTCGTT597490.31524623306103516No Hit
AACGAGGTAAACTAATTTTGATCTTTGTGTTCTCCAGTTCAGTCAAAATG525220.27711530992705635No Hit
CATTTTGACTGAACTGGAGAACACAAAGAAAGATCAAAATTAGTTTACCT465920.2458275869182706No Hit
AACGAGGTAAACTAATTTTGATCATGTCTGCTCCATCTCTACTTTTTTGG462710.24413393445860448No Hit
AACGAGGTAAACTAATTTTGATCTTGAATGATATGAACAACAGTCGGGCT435410.22972997428761205No Hit
CATTTTGACTGAACTGGAGAACAAGATCAAAATTAGTTTACCTCGTTAGA429150.2264270881824687No Hit
CATTTTGACTGAACTGGAGAACACAAAGAGATCAAAATTAGTTTACCTCG398390.21019757115463994No Hit
AACGAGGTAAACTAATTTTGATCTTGTTCTCCAGTTCAGTCAAAATGAGA389320.2054120796253029No Hit
AACGAGGTAAACTAATTTTGATCTTTCTTTGTGTTCTCCAGTTCAGTCAA377330.19908594473701718No Hit
AACGAGGTAAACTAATTTTGATCTCTTTGTGTTCTCCAGTTCAGTCAAAA341620.1802447206452172No Hit
AACGAGGTAAACTAATTTTGATCAGACGAATATCATCTTCTCAAATAAGT320400.1690486754134055No Hit
AACGAGGTAAACTAATTTTGATCGTCAGAATTCACTGCTCAGATGTCTAT319180.16840498195521467No Hit
AACGAGGTAAACTAATTTTGATCAGTACAGTTGAAATATATTTCGAACAC297510.1569715088084965No Hit
CATTTTGACTGAACTGGAGAACACAAAGGATCAAAATTAGTTTACCTCGT282070.14882509323926124No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT281730.14864570325910967TruSeq Adapter, Index 22 (97% over 40bp)
AACGAGGTAAACTAATTTTGATCCTTTGTGTTCTCCAGTTCAGTCAAAAT245430.1294931847899879No Hit
AACGAGGTAAACTAATTTTGATCGGTCATTTTGTAGTCATGAGTAGTACA242420.12790505584805795No Hit
AACGAGGTAAACTAATTTTGATCTGCAAGTGTTTGTATTGGTACAGTTGA238780.12598452782525896No Hit
AACGAGGTAAACTAATTTTGATCAAGTGTGTCGTTCACCCAAATAAGTGT229890.12129400746188453No Hit
AACGAGGTAAACTAATTTTGATCAGGACAGTTTGAGATATTGAAGAAAAT219390.11575402278073357No Hit
AACGAGGTAAACTAATTTTGATCAAGTCCTAAACGCATACCCAGCTAACA217270.11463547349272976No Hit
AACGAGGTAAACTAATTTTGATCTGAATGTTCAAACGGACGTTTTTCGTG214990.11343250539053701No Hit
AACGAGGTAAACTAATTTTGATCTATCGAAGCGGCAAGAAATATATTTTT213370.11257776489687371No Hit
AACGAGGTAAACTAATTTTGATCGAGGTACAGTTGAAATATATTTCGAAC208440.10997661018467618No Hit
AACGAGGTAAACTAATTTTGATCGTTCCTACTGTTTCCAGTACAGTTGAA205740.10855204269523736No Hit
AACGAGGTAAACTAATTTTGATCGCAAGTACAGTTGAAATATATTTCGAA202250.10671065731074054No Hit
CATTTTGACTGAACTGGAGAACAAAGAAAGATCAAAATTAGTTTACCTCG197850.10438913992054397No Hit
AACGAGGTAAACTAATTTTGATCCCTTGTTGAACAAGTATTCGAAACACT195530.10316506711480393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGG8511500.069.579081
ACGAGGT8841250.068.906542
GTACGCC16550.068.7297546
GTACGCT20200.068.612546
TGTACCA1029600.068.57429545
CTGTACC2584200.068.5575844
TGTACTA691850.068.5467445
CTGTACG392900.068.5377144
GTACCGT71200.068.52392646
ACTGTAC8282300.068.5071543
CTGTACT3779050.068.48344444
TGTACGC57500.068.476945
GTACTTC368150.068.4680246
GTACCCG38300.068.44511446
GTACTTA328450.068.44285646
TGTACCC429350.068.4171845
TGTACCG306950.068.402345
GTACGGG141050.068.3857446
TGTACAT513750.068.3840545
TGTACAC265900.068.37961645
Produced by FastQC (version 0.11.2)