FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_dj12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_dj12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21823783
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTC397082018.194920651474586No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATT396417818.16448596469274No Hit
GAAAGTGGTAAGGTTGGAAAAACGTTGTCTTCGCAAGCTTAGATCGGAAG17114817.842274641385501No Hit
AAGCTTGCGAAGACAACGTTTTTCCAACCTTACCACTTTCAGATCGGAAG17075527.824271346539691No Hit
GAAAGTGGTAAGGTTGGAAAAAACGTTGTCTTCGCAAGCTTAGATCGGAA7470023.4228804419472096No Hit
AAGCTTGCGAAGACAACGTTTTTTCCAACCTTACCACTTTCAGATCGGAA7276603.3342523612885997No Hit
AGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCGCA4370512.002636298207327No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG3362971.540965652013677No Hit
GAAAGTGGTAAGGTTGGATAAAAACGTTGTCTTCGCAAGCTTAGATCGGA3311231.5172575717051437No Hit
AAGCTTGCGAAGACAACGTTTTTATCCAACCTTACCACTTTCAGATCGGA3134911.4364649795134052No Hit
GAAAGTGGTAAGGTTGGAGAAAAACGTTGTCTTCGCAAGCTTAGATCGGA3055971.4002934321698488No Hit
AAGCTTGCGAAGACAACGTTTTTCTCCAACCTTACCACTTTCAGATCGGA2475901.1344962511769843No Hit
AAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCGC2205961.0108055051683753No Hit
GAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATT2185661.0015037264620896No Hit
AAGCTTGCGAAGACAACGTTTTTTTATTCTTATTCTTCTCCAACCTTACC2089860.957606662419618No Hit
GAAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAAAAACGTTGTCTTCGC2033680.9318641044038973No Hit
CTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATTAAA1874070.8587282965561012No Hit
GTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCGCAT1840960.8435567747351593No Hit
AAGCTTGCGAAGACAACGTTTTTCCTCATAGCTAATTCAGACAAAAAAGC1341400.6146505397345638No Hit
GAAAGTGGTAAGGTTGGAGAGTGGCTGAAAATGTTTGCAATTTGATTAGT1329780.6093260733026901No Hit
AGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATTA1078340.494112317740696No Hit
GAAAGTGGTAAGGTTGGAGAAGAATAAAACGTTGTCTTCGCAAGCTTAGA916840.4201104822202457No Hit
AAGCTTGCGAAGACAACGTTTTATTCTTCTCCAACCTTACCACTTTCAGA684370.3135890784837807No Hit
GCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATTAA567660.2601107241581352No Hit
TGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATTAAATA541370.24806423340994546No Hit
GAAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAACGTTGTCTTCGCAAG521150.23879911195964512No Hit
GAAAGTGGTAAGGTTGGAGAAACGTTGTCTTCGCAAGCTTAGATCGGAAG501850.22995554895317646No Hit
AAGCTTGCGAAGACAACGTTTTTGGCGTGTTGAAGAGTTTGCAAAGTAAG494560.2266151565015103No Hit
AAGCTTGCGAAGACAACGTTTTTATGACTACTTTCTGTCTCGTGGCTTCT482770.22121279340066752No Hit
GAAAGTGGTAAGGTTGGAAAAACGTTGTCTTCGCAAGCTTAGAAAGTGGT482580.22112573241770228No Hit
TGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCGCATC473110.21678642974043502No Hit
AAGCTTGCGAAGACAACGTTTTTTTTCTAAAGCTGCTATAGTAACCACCG467340.21414252515249074No Hit
AAGCTTGCGAAGACAACGTTTTATTCTTATTCTTCTCCAACCTTACCACT436510.2000157351271317No Hit
GAAAGTGGTAAGGTTGGAGAAGTTGTCCGTAAATACATTAGCAATCATAT430260.19715188700327527No Hit
GAAAGTGGTAAGGTTGGAGAGAGTAGAAGACACATTCACTGTTTTACACT427350.1958184793168077No Hit
AAGCTTGCGAAGACAACGTTTTTCCAACCTTACCACTTTAGATCGGAAGA405100.1856231799958788No Hit
GAAAGTGGTAAGGTTGGAGAAGATTACCGAGCTAAATTGGGTTTCCGTCA399650.183125904431876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG395450.18120139849264447No Hit
AAAGTGGTAAGGTTGGAAAAACGTTGTCTTCGCAAGCTTAGATCGGAAGA376960.1727289902030276No Hit
AAGCTTGCGAAGACAACGTTTTTCCAACCTTACCACTTTCAATTTTCTAA357240.16369297660263576No Hit
AAGCTTGCGAAGACAACGTTTTTCCATTTTTAAACGTCACTGCTTCCATT353980.16219919342123224No Hit
GAAAGTGGTAAGGTTGGAGAAGAAGCTGGGAATTGTGTGGAGCAAGTGGT353880.16215337185125053No Hit
GAAAGTGGTAAGGTTGGAGAAAACGTTGTCTTCGCAAGCTTAGATCGGAA353380.16192426400134202No Hit
GGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCGCA352200.16138356947555793No Hit
GAAAGTGGTAAGGTTGGAGGACAGCTGAACACCAAGTCATGATTTTATAT329640.15104622328768574No Hit
AAGCTTGCGAAGACAACGTTTTTGTCTGAAAGGCACGGTGAGTAAGGGGA329120.1508079511237809No Hit
AAGCTTGCGAAGACAACGTCTTCTTCATTCATTTTGTTAACCTCTGTTAT324290.14859476929366464No Hit
GAAAGTGGTAAGGTTGGAGAAAAAACGTTGTCTTCGCAAGCTTAGATCGG316270.14491987938113204No Hit
GCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATTAAATAA307760.1410204637756891No Hit
GAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG287740.13184698546535217No Hit
TTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATTAAAT286210.1311459154446321No Hit
GAAAGTGGTAAGGTTGGAGAAGACGAGCAGGTGGAATTTCGCGATTTATC274690.12586727058273994No Hit
AGCTTGCGAAGACAACGTTTTTCCAACCTTACCACTTTCAGATCGGAAGA268600.12307673697085422No Hit
AAGCTTGCGAAGACAACGGATAGAGAAGGTAGACTTCTTAAGAGAAACAG264290.12110182730464282No Hit
AAGCTTGCGAAGACAACGTTTTTCCAACCTTACCACTTTCTAAGCTTGCG260280.11926438234837652No Hit
AAGCTTGCGAAGACAACGTTTTTTCTCCAACCTTACCACTTTCAGATCGG244430.11200166350627662No Hit
GAAAGTGGTAAGGTTGGAGAAGAAGCAAAAAAGCTGTAGAAGGAATGAAA229670.10523839977697724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCC207.9278056E-570.007133
CCTTTAG207.932305E-569.99911559
GTTACGC252.8430768E-669.99847442
TACGCTC252.8430768E-669.99847444
GGGCTTG37950.069.638351
AAGCTTG9115300.068.992741
GAAAGTG9417650.068.718311
CAACGCT6600.068.4105814
GCTAGCG14800.068.3515553
GTTAACC37450.068.2228136
CTCCTCG5050.067.91946427
AGCTTGC9569000.067.8747252
TTAACCT37900.067.7821637
TCTGCGG37750.067.6814270
TAACCTC38150.067.3379838
CCTCTGT38000.067.2352241
GCTTGCG9706700.067.1460343
GAGCTTG318850.066.9114761
GGCAAGT12100.066.8168850
ACAGCGA30150.066.6323547
Produced by FastQC (version 0.11.2)