FastQCFastQC Report
Thu 19 Jul 2018
HWK5TBGX5_n01_dj11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWK5TBGX5_n01_dj11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12823632
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAAC440836634.37689103991755No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGC159196312.414291052644055No Hit
GCTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAAC7336675.72121065233313No Hit
ACTTGCAAACACTGGTCAATGAATATATAAGCTTTCGAAACTAGGCTTTG2619592.0427831990188117No Hit
TCAAAGCCTAGTTTCGAAAGCTTATATATTCATTGACCAGTGTTTGCAAG1494531.165449850713121No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACT1364101.0637391965084464No Hit
ACTTGCAAACACTGGTCAATAAGCTTTCGAAACTAGGCTTTGAAGATCGG1054380.822216358048952No Hit
CAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCA1046570.816126039799021No Hit
TCAAAGCCTAGTTTCGAAAGCTTATTGACCAGTGTTTGCAAGTAGATCGG779120.6075657816755815No Hit
GCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACTAAT626510.48855893556521274No Hit
TCAAAGCCTAGTTTCGAAAGCTTAGCACCGACCAGCACAGACAGGCAATG515980.4023665058385955No Hit
ACTTGCAAACACTGGTCAATATAAGCTTTCGAAACTAGGCTTTGAAGATC455870.35549211019155885No Hit
ACTTGCAAACACTGGTCAAGCTTTCGAAACTAGGCTTTGAAGATCGGAAG412790.3218978835325281No Hit
TCAAAGCCTAGTTTCGAAAGCTTGACCAGTGTTTGCAAGTAGATCGGAAG406420.31693049207899915No Hit
ACTTGCAAACACTGGTCAATGAGTAACAACTCGATATGAAGCTACAGAAC396810.3094365153335654No Hit
TTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACTA393370.30675396798660476No Hit
TCAAAGCCTAGTTTCGAAAGCTTATATTGACCAGTGTTTGCAAGTAGATC325140.25354751290430044No Hit
TCAAAGCCTAGTTTCGAAAGCTTTCAAGAAATCCGCGTTAGCGTAGTAAA291660.2274394648879506No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTTTTTCCTCTGTGTATAGAGCATCTG283540.221107405452683No Hit
AAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAA276560.21566432973123373No Hit
AAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAAC272660.21262306965764455No Hit
TCAAAGCCTAGTTTCGAAAGCTTCAAACCCAACCATCTCGGATCAAAAAT256470.19999794130087326No Hit
TGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACTAA244010.1902815052708936No Hit
ACTTGCAAACACTGGTCAATAAGCTTTCGAAACTAGGCTTTGAGATCGGA235410.18357513690349192No Hit
ACTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACT222250.1733128336808168No Hit
ACTTGCAAACACTGGTCAATTAAGCTTTCGAAACTAGGCTTTGAAGATCG208330.1624578746489294No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTTGTACTAAAATTTTGTGGCGAAATT191550.14937265822974333No Hit
AGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACC179540.14000713682363936No Hit
GCTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGCAAC177720.1385878821226311No Hit
TCAAAGCCTAGTTTCGAAAGCTTAATTGACCAGTGTTTGCAAGTAGATCG161100.1256274353474897No Hit
ATTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACT159290.12421597874923422No Hit
TCAAAGCCTAGTTTCGAACTAAAATTTTGTGGCGAAATTTGAATACACAT153460.1196696848443561No Hit
ACTTGCAAACACTGGTCAATGAGCTTTCGAAACTAGGCTTTGAAGATCGG150950.11771236105340514No Hit
ACTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGCAAC150630.11746282176531579No Hit
ACTTGCAAACACTGGTCAATGCCTGTCTGTGCTGGTCGGTGCTAAGCTTT148870.11609035568082428No Hit
ACTTGCAAACACTGGTCAATGAAGCTTTCGAAACTAGGCTTTGAAGATCG144230.11247203600352848No Hit
ACTTGCAAACACTGGTCAATGATATTAAGCTTTCGAAACTAGGCTTTGAA142090.11080324201443086No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACGTAGTTACAGAAATAGTACTAAAA140820.10981288296482619No Hit
ACTTGCAAACACTGGTCAATAAATTTAAGCTTTCGAAACTAGGCTTTGAA139010.10840142636657073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGCA6282350.069.676541
CTTGCAA7398950.067.766612
TCAAAGC4430950.067.722281
GCTTGCA939300.067.016631
TTGCAAA7489250.066.889243
TGCAAAC7568300.066.303484
GTCAATA337000.065.91820515
CTAATTC5806100.065.4724250
GCAAACA7668050.065.468365
CAAACAC7669450.065.450196
AAACACT7655150.065.445067
ATTCATC5752700.065.4411558
GTATTTG5872400.065.42808529
TAATTCA5806350.065.427551
GGTATTT5873650.065.4254828
TGGTATT5875250.065.4052827
TCATCAT5761350.065.4027960
CATCATT5758800.065.4019661
CATTTAA5785050.065.3944964
AAGAAAC5783200.065.3885344
Produced by FastQC (version 0.11.2)