FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34901971
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9153522.6226369851719835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC139500.034.1079063
GAGCAAA525300.029.3052861
GCAAAAG519500.027.603743
CAAAAGC600800.025.1511024
GAGCGAA94000.024.3606871
AGCGAAA226550.020.411081
AGCAAAA760150.019.7669452
AAAGCAG954600.018.2844896
AAGCAGG949900.017.8386217
GCGAAAG276650.016.8246672
AAAAGCA1001300.015.9822855
GCAGGTA208750.015.85269459
CAGTACG61800.013.826339
TACTAGA185650.013.5591224
GTATACT190050.013.1761191
CTATGCT162750.013.1424644
ATACTAG188800.013.1250013
TATACTA201100.012.948232
CTAGAGT187250.012.9238546
GTATATA168000.012.8389421
Produced by FastQC (version 0.11.5)