FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_Mirella_HA_sample_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_Mirella_HA_sample_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACAATCGAAAGAAAAGACCGACTATTATAAAATCCCTAAGCATAATCA10.9259259259259258No Hit
GAATGGTAACACGCTTCTAGTCTCTGATAAAATACAGAGATTAGAATCGA10.9259259259259258No Hit
AAATATCAGCATGCGAGGTAGAGAGAGCGAGAATCAGTCGCCAAGCAGCG10.9259259259259258No Hit
AGCCTGCACAATAATTAGCAGTTTTCTTTACTATTCTGAAAAGCCTTAAA10.9259259259259258No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACAATTACCTTCTCTT10.9259259259259258No Hit
CAATGGTTGTTTCAAAATATACAACAAATGTCACAATGACTGCATGGGGA10.9259259259259258No Hit
TAATAGACCCTCTATGCGAGAGATTGGACCAGGCGATAATGGAAAAGAAC10.9259259259259258No Hit
GTAATACTCAACAACATCGAATAAAGCACAAGCAATCTAAATACTGCTTA10.9259259259259258No Hit
ATACAGAGATTCGCTTGGATAAACTGTGATGAGAATTGGAGACCTTCACT10.9259259259259258No Hit
TGCCAACCGTGTTGCCTCTTCCATATAAGGACATTTCATCACGGCGGAGC10.9259259259259258No Hit
ATTCACTATTAATTGATGGCCATCCTAATTCTTTTGGTCGCTGTCTGGCT10.9259259259259258No Hit
ACAACGGTGTATTTTATCATAAAATAAGCAGAAACGAGAAATATCTTATC10.9259259259259258No Hit
AAAAATATCATTAAGTCAAAAAATCAGAAAGTATAATCAAAATAGGACTA10.9259259259259258No Hit
GTTCAGGATCTTAGGGTCAAACTCGAACATGGGCCCTTTGATCTTGGGGG10.9259259259259258No Hit
GGGAATCAGGAATCTGCTCACATGTTGAACACACCAGGCCAAATGCCACT10.9259259259259258No Hit
GTCCTATCACGTGCTGTGTTATTTGAATGCACGTTGTATATTGTTGTTCC10.9259259259259258No Hit
GACATATGATACCAAGACTTAGTAGAAGTCTTAGACAAAATACTTATTTA10.9259259259259258No Hit
GTATTAATAGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG10.9259259259259258No Hit
GACCTAAAAAGTAAGATGAAACACCCTTGGCCTCGATATCGAAACAGCCA10.9259259259259258No Hit
ATATTTAAAATATACTAAATTTAAAAATGTAAACAAAAGAAAATGAACAT10.9259259259259258No Hit
GGTAAGGGGAATGCTCCCCGCAGATATTGAGGAGTACACCTAAAGATCCA10.9259259259259258No Hit
GTAATATCCTGTTCTACATCAGGCAGTACTTGTCAGGCTTGCATAAATGT10.9259259259259258No Hit
CCACAATGGGCCATTCTTATGATTTATTAACAAAAAACACTTAGGACATG10.9259259259259258No Hit
GAGCAAAAGCAGGGGGAGTGGAGGCAGAAGAAGCAGGTTCACCACTGAGC10.9259259259259258No Hit
CTCTTATCTATACTTCTACTACAAAAAGTATTTTTAATCATTCAATAAAT10.9259259259259258No Hit
TTAATAATGTTGCACATTCTCTCATAAGCACTTCTTGGTTTGCGCCCAGT10.9259259259259258No Hit
CCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCT10.9259259259259258No Hit
ATAAAGTAGAAAATATTTCATAGGTACAGGTATGACATTCATAATTAATA10.9259259259259258No Hit
CACAAAAAGCCATTATAAGAATAAAGATCAAAAAACGCGAAAATAAAAAA10.9259259259259258No Hit
CTTCTTGCTCTGAGGATTTGCAAGTCTATGAAAAAGAATTTTATCACTTG10.9259259259259258No Hit
AAATTTAACACTAAACTCCTCAGGCAACGACTAATGTCTTTCACTGAGGA10.9259259259259258No Hit
CACTTAGAATGACAATTGCATATGAACCTACTTCGCGCTTCCTTTCTGAC10.9259259259259258No Hit
GTGTGAACCAACTATAATAGAAAGAAACATAAAAGAGATAGTGTATTTGA10.9259259259259258No Hit
GTGTAGGACAGCATGCAGTCCACGCTCATGGAATCCCGACCGGTAGCATG10.9259259259259258No Hit
CTGTGTCCATGTACAAGCTGTCTAGCCTCTCTGAGTGTCAGAGGTTCTCA10.9259259259259258No Hit
GTGATACTTTTTACAACTGTCCAGGCCATTGTTCTGTCTCTCCCCTCGAT10.9259259259259258No Hit
ATCAATTCCACCAATTGTTTCCAAACAAGACTGGCTGTTACGATATCGAG10.9259259259259258No Hit
GGAACAGATAGAGTGATGGTATCACCTTTGGCTGTAACAAGAAGACGGCA10.9259259259259258No Hit
GATATAAATGATGCTGTGCATTAAAATTTACACACCAATAATCCACGAAT10.9259259259259258No Hit
CTAATTCAATCAGTAGTTGTAAGGCTGGCAGAAATGTTATGTGTTCTAAA10.9259259259259258No Hit
CCTCAAAGATTAAACAGTACATTCAGTGTAAATATTAATATACACACACA10.9259259259259258No Hit
ATGGTAGTGTTTGTTTTCAAGAGCACAGTGCCCATGGAGCGATGACATCA10.9259259259259258No Hit
CTCTGAAATTGGCAACAGGAAGGCGAAATGTACCAGAGAGACAAACTAGA10.9259259259259258No Hit
TATGTCTCCAGGGCAAAACTTAACCTAAAGTCGATTGAGAGATATTAATA10.9259259259259258No Hit
AATTCGGACAGAAATTTGAGGAAATAAGGTTGTTAATTGAAGAAATGCGG10.9259259259259258No Hit
GAATTGTGCAGGTGTGCGGTACTCCCGCCAACTTGCCGAGGTCAGTGGTA10.9259259259259258No Hit
CCTCAAATTTCTGTCCCAATTGCTCTCGCCATTATTCATTTCTGCTCTGG10.9259259259259258No Hit
GATTTTTACCATAAACAAACCCTGCATTAAAAAACAAAGATGCATATACC10.9259259259259258No Hit
GGTTGGTATCGTGGTATTATGCATATCTGGGGATGTGCTAAATGCAGCTT10.9259259259259258No Hit
CTCATGATCTGAGCTGAAGGCAGCTGATAAACTGACTGAAGCCCCCAGGC10.9259259259259258No Hit
TCCATAAATAAAACATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTA10.9259259259259258No Hit
ACTTAAAAAAACCACCTTATTTCCTCAAATTTCATTCCCAATTGCTCTCT10.9259259259259258No Hit
AGCGAATTGTTAGTAGGATGCTGTTGAGCTTTGGCGGAGCACCATTAGTT10.9259259259259258No Hit
TCCCAATAGAGCATCTATAAGTGTGCAGTTTTCTCCATCAAGGATCTGAT10.9259259259259258No Hit
TTTCAACTCCCTTATGCAATCTTCATAAACATAATGGACTCTAATTAAGT10.9259259259259258No Hit
GTTTTCACGAAGACAAAACAGCAGTTGGGGTCCTCAAGCGAGGACTAGAA10.9259259259259258No Hit
AGAGTGAGCTATTAAAATTCACAAAGCTATATGAAGAAATACCATAACTT10.9259259259259258No Hit
GAGCAATAGATGGAGAAATTTAACCATTACCTACTATACCAGGACATTCT10.9259259259259258No Hit
ATTAGCTACCTGGAAACTAATAAGGGATTTCACACAAGATGGAACAAAAG10.9259259259259258No Hit
GAGAAATAAAGATAAAAATTACANTNGNNANNCNNNNNNNNNNNNNNNNN10.9259259259259258No Hit
GATTTAATCTTATTGGCCTTTTCAAGGTAATATATGTGGACTTCTCTCCT10.9259259259259258No Hit
GAATAGTAACACTCATAGAGTCTCGGAAAATAAACCGAGATTCTGTTGTA10.9259259259259258No Hit
TTTCTAAACTATTATCTGTCCCTATAAAGCTGTGCCCCAATTCTGCAATT10.9259259259259258No Hit
TCAAAGGTCAAAATGAAATGGGGAATGGAGATGAGACATTGACTCCTAAA10.9259259259259258No Hit
GTTCAAAACCAATCATGTTTATACATACTTCCCAAACTCATAAAGGCGAA10.9259259259259258No Hit
GGTGAAAGGGTACGCATAAATAAAAGACAATACAGATAAGAACATAAGCA10.9259259259259258No Hit
AGTAGAAACAAGGTAGGTTTTGACTCCAACTCTATGAGGTCAAAATGAAT10.9259259259259258No Hit
GTCCCTTGTCAGCACGGGGAGCCCCTTTCTCTCTCTTCTACTGCCGTCTG10.9259259259259258No Hit
AATAACATAAAGTAATTGTGTTGAAGATGAAACGATAAATGTTTTCATAT10.9259259259259258No Hit
GATTAAAAGCTTTGTCAATGAGATAGCGGTCTAAATGACGAAAGAGGGCA10.9259259259259258No Hit
GCACAGATCAGGATGGACCCTCTCTGGAGGTATACCAACTGGAGCCTCTG10.9259259259259258No Hit
TCTCTGAAAATATACAGACACTAGAAACGAGTTAAACATCCAAACAATTC10.9259259259259258No Hit
GATTAATTCTGGGAAGTCTCTTCTGTCTTCGTTAACTATAATGCGGAACA10.9259259259259258No Hit
ATGCTCGTCCGTTTATTCTCTCTTCTTTACCTATAATGAGGAACCCTCTC10.9259259259259258No Hit
GCAAAATTCTTCTAAGAACCACAAACATTTGAACTAGTAATTTTAATCCA10.9259259259259258No Hit
GTTGTAACGCTTGCATAAATGTTATTTCATCGAAACTATTCTCTCTCGCT10.9259259259259258No Hit
GTCAGAATAAGAAGGTTTTTTTGGTTGCTTTTTGTTGTTTGTGTTTTCAT10.9259259259259258No Hit
AGTTATTTGTACTAAACTAATCTCTGTCTCTTTCAATCTGTGCATCATTT10.9259259259259258No Hit
TAGTACAAACAAGGGTGTTTTTTATCATTAAATAATCATAAACAAGAAAT10.9259259259259258No Hit
TCCTTGTTCTATGCAGACAGTAGGGTCCCAGCTCTAGCCATTTTATAATC10.9259259259259258No Hit
GATCATTAAAGAAGAATCCAGCGAGACAATTAGAATTAAAATAGCATCTG10.9259259259259258No Hit
GGGAGAAATTGAAAGATTAGCCTTCTAACCGAGGTCGAAACGTATGATCT10.9259259259259258No Hit
AGCTAATAGAGTCCTACAGCCTTTTCCAATGGAACCTTCTTCCACTCCTT10.9259259259259258No Hit
GCTCAGAGCCTGGCGAATGATATCTCTTCCTGAAGGGTGTACTGAATGAT10.9259259259259258No Hit
GGTAAAGCACAGATTTGAAATAATCGCGGGGAGCGACAGAACAATGGCCT10.9259259259259258No Hit
TCTCCAAGAAGAGGAATATCAGACTGGGCAGTGTCTCCAGCACCTTCAGG10.9259259259259258No Hit
CCTTAAAATATTGAGATTCTGGCTTTTAAAAAAGAAGCTTGCCAACCCAA10.9259259259259258No Hit
TAAAAAGACTTGCAAATAAGTTATTTCTTCCAAACTATACTCATTACATA10.9259259259259258No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTATAAGGCCGTGTTTGAAGA10.9259259259259258No Hit
GACTCATAATAGTTCAAGTAATTGTATAAGAGATGATATTATTTAAAATT10.9259259259259258No Hit
GACTCGAGTATTTCCCTCATGTTCACTCCCACGGTTAGTGTGCCCACCCC10.9259259259259258No Hit
AAACTATTCTCTGTCGCTATCAATCTGATCCCCATTTCTTCAAATACACA10.9259259259259258No Hit
GCCACATACCTAGATCGGCTCCGCCGATATCAAAACACCAAAAAAGGAAG10.9259259259259258No Hit
CATCTGGGTCTTCAATTAACCATTTCTGTTATCCTTTTGTCAGCAGTGAT10.9259259259259258No Hit
CTCCTCAATTGCTTCATATAGCCACCCCAGATCAAAGGTCCCAGGTTCGT10.9259259259259258No Hit
CTAAAGGTACAATCGCACCCAATTAGGCCCTATTATCTTTTGCCTAGGCA10.9259259259259258No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT10.9259259259259258No Hit
TCCATGAGTTAGCCCGCTTACGGATGTCATGTGATCAAATGAAGCTCACA10.9259259259259258No Hit
ACCCCTAACAGACCAAGCAACACAGGCAGCCCTTAGAGCCAACTTAAAAA10.9259259259259258No Hit
TATCTATTCTGAAGCAGTTTTGGATCTACCCTACTTCATGATCACAGAGT10.9259259259259258No Hit
GTGCAAGATCCCAATGATATTCGCGGCACCAATAAGCGGATCACATGATT10.9259259259259258No Hit
GTTTTCTTGACTCTGCTGAAAATCCTTCCAATAGTGGTGATGCATACAGG10.9259259259259258No Hit
GATCATTACTATTTGCAATGTCTAATTTGATTGAGTAACATATCAGCCAG10.9259259259259258No Hit
GTCACGTCCACAACGTTTCAGAGGAAATACAGCAAATTCTAACATATATA10.9259259259259258No Hit
TATACATAGATTCTCATGGAGAAACTGAGATGAGAATGTGAGACCTTAAC10.9259259259259258No Hit
CAACAGTTAAACACCATAGTAGTATCAATGTCTCTCATATGTAAAAGATT10.9259259259259258No Hit
ATTGAAGCCGAGTCCTCAGTTAAAGAAAAAGACATGACCAAAGAGTTTTT10.9259259259259258No Hit
CATCTGATTTATTCTCAAAAATCTCTTTGGTCATGTCTTTTTCTTTAATA10.9259259259259258No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers