FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_Blank_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_Blank_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268589
Sequences flagged as poor quality0
Sequence length151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCC71742.670995461467149No Hit
GTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCC56602.1073089367025455No Hit
GGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTC56272.0950225065062233No Hit
GGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGC56112.08906544944134No Hit
GAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTC43511.6199472055817625No Hit
GTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTT42311.575269277595136No Hit
CCCCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGG41311.538037670939614No Hit
CCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTA35641.3269344612028044No Hit
AAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGG31521.1735402417820535No Hit
CCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTAG29151.0853013340084665No Hit
CGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGG28421.0581222611499355No Hit
GTATTAAAGATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGA27541.0253584472930761No Hit
AAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTAC27011.0056256957656493No Hit
GTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATA25460.9479167054495903No Hit
GTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGT24080.8965370882649698No Hit
CCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGA23290.8671241190071075No Hit
GTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGA22840.8503698960121227No Hit
AGCGAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACG21950.8172337660887079No Hit
GATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCC21430.7978733306278365No Hit
GGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACG20920.7788852112335204No Hit
ATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCG20560.7654818328375325No Hit
TTCGAGCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTTG18660.6947417801920406No Hit
GCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTT18630.693624831992375No Hit
GCGAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGC17930.6675627073335095No Hit
GTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGAACGAA17670.6578824896030738No Hit
GTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCC16530.6154384580157788No Hit
ACGTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAA16410.6109706652171161No Hit
GTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCT15410.5737390585615941No Hit
AGCGAAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTT15370.5722497942953733No Hit
ATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAA13690.5097006951140962No Hit
GAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGG13690.5097006951140962No Hit
GTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCT12760.47507530092446076No Hit
AGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGG12640.47060750812579816No Hit
GAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAAGATG12580.46837361172646685No Hit
CCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTG12570.46800129565991155No Hit
GAGCAAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCAC12500.46539508319402506No Hit
GCAAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGC12360.46018265826225196No Hit
GAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGC11970.4456623316665984No Hit
GTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAA11620.43263126933716567No Hit
CTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGG11550.4300250568712792No Hit
GGTTCGAGCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTT11240.41848325880806736No Hit
GCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGG11220.4177386266749569No Hit
GTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACG10920.4065691446783003No Hit
CCTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATACAAAAGCC10640.3961442948147541No Hit
TCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTG10600.39465503054853324No Hit
GCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCACG10600.39465503054853324No Hit
CTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGT10580.3939103984154228No Hit
TCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATAC10200.3797623878863245No Hit
GGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGG10150.3779008075535484No Hit
AGCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCG10120.3767838593538827No Hit
TTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATT9710.36151890062511866No Hit
GTCCAGAAGCTTACCAGGGAAGGGAAGAAAAAGCATGGTTTCAGTGCCAT9550.3555618435602351No Hit
ATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTG9420.3507217346950173No Hit
CAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGT9220.3432754133639129No Hit
CGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCG9220.3432754133639129No Hit
CGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGAACGAAGCCC9150.3406692008980263No Hit
GTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCCT8880.33061666710103543No Hit
GTGTTACTCTGTCCAGAAGCTTACCAGGGAAGGGAAGAAAAAGCATGGTT8660.32242571363682054No Hit
AACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAAGATGT8500.31646865657193707No Hit
CTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCCTTCGTCTAGAGGCC8490.3160963405053818No Hit
AGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAAC8280.3082777031077222No Hit
GAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCG8050.29971443357695216No Hit
ATTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGA7850.2922681122458477No Hit
TTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTTCCAGTTAGCTTACCCC7780.2896618997799612No Hit
GCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAA7420.2762585213839733No Hit
AAGCAGTACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCA7390.27514157318430765No Hit
ATTAAATAGAGGTAATAAAGGCCATCAGAATTCCAGAATGGGAGAACTTA7260.2703014643190898No Hit
GGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCT7240.2695568321859793No Hit
CCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTG7160.2665783036535376No Hit
GCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGG7070.2632274590545406No Hit
CATCATATGATTAAATAGAGGTAATAAAGGCCATCAGAATTCCAGAATGG7060.2628551429879854No Hit
GTTCGAGCCCCGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTT7020.2613658787217645No Hit
GGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGA6920.25764271805621225No Hit
TTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCG6640.2472178681926661No Hit
CCGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTA6630.2468455521261109No Hit
CAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGCC6590.24535628785989No Hit
GTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACG6480.2412608111277826No Hit
ACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAG6400.23828228259534082No Hit
GTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAA6120.22785743273179468No Hit
GATTAAAACAGAGAGCAATTGCTCTGGAAAATAAAGGTTGGCAGTTATTA6110.22748511666523946No Hit
AGCAAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACG6100.22711280059868424No Hit
TCCCAGGACTCTGGGATCATGACCTGAGCTGAGGGCAGACGCTTAACAAC5660.21073089367025455No Hit
CCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCC5560.20700773300470235No Hit
TAGTTGGACTGATGAGATTAAAACAGAGAGCAATTGCTCTGGAAAATAAA5480.20402920447226058No Hit
CACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTAC5460.20328457233915015No Hit
CTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGAACG5180.19285972247560398No Hit
AATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGG5070.18876424574349657No Hit
GCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGG4990.1857857172110548No Hit
GCGAAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTC4900.18243487261205782No Hit
GAGCGAAAGCAGGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCAC4900.18243487261205782No Hit
ATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAG4840.1802009762127265No Hit
GCCTAGGACATCACCCTTTCACGGTGAGTACAGGGGTTCGAATCCCCTAG4830.1798286601461713No Hit
ACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTGGCGGAGCG4780.17796707981339518No Hit
GCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGC4770.17759476374683997No Hit
TTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGT4730.1761054994806191No Hit
CACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACC4690.1746162352143982No Hit
GGACAACGTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTC4650.17312697094817733No Hit
GTACGTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCACGGTG4640.17275465488162212No Hit
TGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCT4630.1723823388150669No Hit
GGAGCGGTAGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCG4610.17163770668195644No Hit
AGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGA4560.16977612634918035No Hit
GTGCCATACATTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTTCCAGTT4540.16903149421606992No Hit
CGTCCGCTCCGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTT4310.16046822468529984No Hit
GGGGACAACGTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACG4270.15897896041907897No Hit
GGATAGCACTTGATCATACATGAGTCAAGGAGGAATGGGAGGAGTGGGAA4260.15860664435252375No Hit
GTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATCTT4220.15711738008630285No Hit
CATATGATGTGTTCAATTAAATCTTACCAATGTTGAAAGGCACCATAGTG4210.15674506401974764No Hit
ACATTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTTCCAGTTAGCTTAC4170.15525579975352677No Hit
GGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAAAGGGTGATG4080.15190495515452979No Hit
GATATCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCT3950.1470648462893119No Hit
AGACGAAGGGGACAACGTACTGCTTACATCTTTAATACAAAAGCCCGCTC3930.14632021415620147No Hit
TCATATGATTAAATAGAGGTAATAAAGGCCATCAGAATTCCAGAATGGGA3900.14520326595653582No Hit
GCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTACTCACCGTGAA3830.14259705349064927No Hit
ACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCGAACCCCTGTAC3820.14222473742409408No Hit
GCCTGTCACGCCGGGGGTCGCGGGTTCGAGCCCCGTCCGCTCCGCCAGAA3790.1411077892244284No Hit
CTATTTAATCATATGATGTGTTCAATTAAATCTTACCAATGTTGAAAGGC3660.13626768035921052No Hit
TTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTC3660.13626768035921052No Hit
CTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGACGAAGGGGAC3580.1332891518267688No Hit
GCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAAA3570.13291683576021354No Hit
AGTTCAGTTGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCG3570.13291683576021354No Hit
GGGTGATGTCCTAGGCCTCTAGACGAAGGGGACAACGTACTGCTTACATC3540.13179988756054792No Hit
GAATGGAAGAGGGAGAAGCAGGCTTCTTAATGAAGGCCTGATCCCAGGAC3410.12695977869533004No Hit
CGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAATC3390.1262151465622196No Hit
GTACATAGACACTGACATCAGATTATCTGAGTTTGAATCTCAGCTCTGTC3360.12509819836255395No Hit
CTCTAGACGAAGGGGACAACGTACTGCTTACATCTTTAATACAAAAGCCC3320.12360893409633306No Hit
GACAACGTACTGCTTACATCTTTAATACAAAAGCCCGCTCAAAAACGTCT3320.12360893409633306No Hit
CGTTGCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGA3300.12286430196322262No Hit
GCGACACTTCTACGAAACGCCGCTGAATCTGGCGTCCCCTAGGGGATTCG3280.12211966983011217No Hit
CGCCAGAACGAAGCCCGTTCAAGACGTTTTTGAGCGGGCTTTTGTATTAA3230.12025808949733607No Hit
ATACATTACAGAACTTCCCGCTGGGAGCTGTCTGTGGTTCCAGTTAGCTT3210.11951345736422564No Hit
GTCTATGTACTTAACACTATGGTGCCTTTCAACATTGGTAAGATTTAATT3190.11876882523111519No Hit
TCGTCACGTTCCTGCCTGCGTTGCGACACTTCTACGAAACGCCGCTGAAT3180.11839650916455997No Hit
CCATAGTGTTAAGTACATAGACACTGACATCAGATTATCTGAGTTTGAAT3170.11802419309800477No Hit
TCTTTAATACAAAAGCCCGCTCAAAAACGTCTTGAACGGGCTTCGTTCTG3170.11802419309800477No Hit
ACACTATGGTGCCTTTCAACATTGGTAAGATTTAATTGAACACATCATAT3140.1169072448983391No Hit
ACGTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCC3130.11653492883178387No Hit
TTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCCTT3100.11541798063211821No Hit
GTTGTCCCCTTCGTCTAGAGGCCTAGGACATCACCCTTTCACGGTGAGTA3030.11281176816623167No Hit
GAGCGAAAGCAGGCAGGATATCGTCACGTTCCTGCCTGCGTTGCGACACT3010.11206713603312123No Hit
TTCGAACCCCTGTACTCACCGTGAAAGGGTGATGTCCTAGGCCTCTAGAC2980.11095018783345559No Hit
GCACTTGATCATACATGAGTCAAGGAGGAATGGGAGGAGTGGGAACCAGA2900.10797165930101382No Hit
GTGTTAAGTACATAGACACTGACATCAGATTATCTGAGTTTGAATCTCAG2880.10722702716790339No Hit
AAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGAT2830.10536544683512727No Hit
CTTAACAACTGAGCCACCCAGGCACCCAGAGAGGCTGTGATTTAACCAAG2810.10462081470201684No Hit
CGTTTTTGAGCGGGCTTTTGTATTAAAGATGTAAGCAGTACGTTGTCCCC2780.10350386650235117No Hit
GTTTTAATCTCATCAGTCCAACTAAGTTCTCCCATTCTGGAATTCTGATG2780.10350386650235117No Hit
GATTTAATTGAACACATCATATGATTAAATAGAGGTAATAAAGGCCATCA2770.10313155043579596No Hit
GTACAGGGGTTCGAATCCCCTAGGGGACGCCAGATTCAGCGGCGTTTCGT2760.10275923436924074No Hit
GTAATAAAGGCCATCAGAATTCCAGAATGGGAGAACTTAGTTGGACTGAT2740.10201460223613029No Hit
GTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTT2730.10164228616957507No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCG100.007080292145.052722
TACTTCA100.007080292145.052722
TTTCCCT100.007092133144.9717145
ATCGGTA100.007096083144.94477
TCGGTAG100.007096083144.94478
GAGCGAA1200.0139.112551
GAGCAAA2500.0124.8383941
AGCGAAA5750.0114.866471
TGCCACA203.8659453E-4108.72877145
ATCCCCA650.0100.36502145
AGCAAAA3450.090.395172
GGACATA1450.089.98244145
TCTGACC651.6370905E-1189.21335145
GCTGCCC651.6370905E-1189.21335145
CGTCTAA259.3864946E-486.98301145
AGGACAA1850.082.281235145
GCGAAAG8350.079.040712
CAGGTCT1050.075.9234164
CGAAAGC9050.072.872753
TACCGTC300.001931406372.526372
Produced by FastQC (version 0.11.5)