FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d8_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d8_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3790840
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG14950.052.3620152
CGAAAGC16300.048.4558943
AGCGAAA18100.042.8856121
TGACGCG3451.7475359E-618.909752145
GTATATA34750.018.5798241
TACACCG5151.6061676E-816.8840275
GTATAAT32450.016.0962311
ATGCGTA4151.0189749E-515.7147667
CAGTACG3254.4703908E-415.6066964
TATACTG24150.015.6022845
AATACCG3757.5516684E-515.4582645
GTATAGA29850.015.0679351
GAGCGAA10650.014.9857841
ACCGTGC8551.6370905E-1114.4077538
GTCTAGA26950.014.2666991
ATACCGT4151.789096E-413.968686
TTTATAC46700.013.9647563
TATAATA40100.013.9181062
GTATTAT40150.013.9127061
GCGTATA3650.001076994213.8972889
Produced by FastQC (version 0.11.5)