FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d8_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d8_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394874
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2600.041.8110353
AGCGAAA2450.038.498651
GCGAAAG2500.037.7047772
ATATAAC2353.45608E-1133.923283
TATAACA2151.8113496E-830.3373054
GTGTTAT1306.4472086E-427.9058271
CAGTGCG1050.007717191227.6085577
GGCCCGT1100.00968254926.353628
GGCCTAT1100.00968254926.353626
GTCTATA1450.001219707925.0190161
GTATAAA2852.7497117E-722.9121511
CCTATCC1650.002603836621.9613513
GTATATA2353.680204E-521.6121731
TTTAGAC1700.003096336321.3154283
ATATATC2404.3670654E-521.13783
ATAATAC2755.2146297E-621.0828973
TATAATA2505.956962E-520.3025722
GCATAGG1800.0042836320.1542071
TCTATAC3351.3150748E-619.4702133
CCTAGAC2256.8751647E-419.3259893
Produced by FastQC (version 0.11.5)