FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d6_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d6_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608665
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC7350.058.1661033
GCGAAAG7250.058.0067942
AGCGAAA7600.056.329791
TTGGACG2405.3065378E-827.1712474
GTATAAG8150.023.1481021
CCGACGC2806.109227E-620.70834145
ACCGTTC3157.3184856E-720.705128
AAGGTCG2455.1339557E-520.7051189
ACGTTAG1750.003670403320.7038337
GTTATAC6950.019.8095263
TTGATCG3001.1089522E-519.327784145
TATTCCG3001.1113349E-519.3229775
TATACTG11500.018.9029125
ACTGGTC12100.018.566168
GTATAGG10450.018.0533051
GTATTAG7650.018.0215361
TATACAG14650.017.8061565
TAGTACT8200.017.6723544
ACACCGT5751.8553692E-1017.6427176
GTCCTAT12150.017.3189451
Produced by FastQC (version 0.11.5)