FastQCFastQC Report
Thu 23 Jan 2020
HWJ22BGXC_n02_2606_d6_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWJ22BGXC_n02_2606_d6_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193492
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG19250.053.4710542
CGAAAGC20300.051.0439953
AGCGAAA22200.045.7427181
GTATATA26300.027.0282081
CGGATCG1100.00968601326.357317145
TACGAAC2259.160831E-725.7639339
TATATCG2353.7292295E-521.583135
TATATAC25500.020.1754323
GTATAGG10600.019.844451
GCTATAT9200.019.7105661
CCTATAC11400.019.7043233
TATACGA3751.8221544E-719.3223447
GCCGACC4155.2647556E-717.46569145
TATACCA34300.017.110995
GTCCATA18800.016.5904251
GTCCTAT22100.016.4106061
TACCATG25850.016.2576777
ATACCAT34450.015.9851026
TATACTA15050.015.8877255
TAGTACC13300.015.7985434
Produced by FastQC (version 0.11.5)